Construct: shRNA TRCN0000101520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153389.2-4711s1c1
DNA Barcode:
GCAGAGTTTAGGAACTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp10d (231287)
Gene Description:
ATPase, class V, type 10D
Transcript:
RefSeq NM_153389.2 (NON-CURRENT)
Match location:
Position 4711 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTTTAGGAACTGGAAT
Hairpin Sequence:
5'-CCGG-GCAGAGTTTAGGAACTGGAAT-CTCGAG-ATTCCAGTTCCTAAACTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTTTAGGAACTGGAATCTCGAGATTCCAGTTCCTAAACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTTTAGGAACTGGAATCTCGAGATTCCAGTTCCTAAACTCTGC-3'

Other clones with same target sequence:

(none)