Construct: shRNA TRCN0000101553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177236.3-916s1c1
DNA Barcode:
CGAGGTCTTCAAAGCCGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp2b3 (320707)
Gene Description:
ATPase, Ca++ transporting, plasma membrane 3
Transcript:
RefSeq NM_177236.3 (NON-CURRENT)
Match location:
Position 916 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGTCTTCAAAGCCGTATT
Hairpin Sequence:
5'-CCGG-CGAGGTCTTCAAAGCCGTATT-CTCGAG-AATACGGCTTTGAAGACCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGTCTTCAAAGCCGTATTCTCGAGAATACGGCTTTGAAGACCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGTCTTCAAAGCCGTATTCTCGAGAATACGGCTTTGAAGACCTCG-3'

Other clones with same target sequence:

(none)