Construct: shRNA TRCN0000101605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007511.1-4693s1c1
DNA Barcode:
GCCTTACAGTGTGTGGTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp7b (11979)
Gene Description:
ATPase, Cu++ transporting, beta polypeptide
Transcript:
RefSeq NM_007511.1 (NON-CURRENT)
Match location:
Position 4693 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTACAGTGTGTGGTGTAT
Hairpin Sequence:
5'-CCGG-GCCTTACAGTGTGTGGTGTAT-CTCGAG-ATACACCACACACTGTAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTACAGTGTGTGGTGTATCTCGAGATACACCACACACTGTAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTACAGTGTGTGGTGTATCTCGAGATACACCACACACTGTAAGGC-3'

Other clones with same target sequence:

(none)