Construct: shRNA TRCN0000101625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025494.1-1129s1c1
DNA Barcode:
GCCGAATAAGAAGTCAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v1c1 (66335)
Gene Description:
ATPase, H+ transporting, lysosomal V1 subunit C1
Transcript:
RefSeq NM_025494.1 (NON-CURRENT)
Match location:
Position 1129 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAATAAGAAGTCAGTGAA
Hairpin Sequence:
5'-CCGG-GCCGAATAAGAAGTCAGTGAA-CTCGAG-TTCACTGACTTCTTATTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAATAAGAAGTCAGTGAACTCGAGTTCACTGACTTCTTATTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAATAAGAAGTCAGTGAACTCGAGTTCACTGACTTCTTATTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000323683