Construct: shRNA TRCN0000101665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008740.2-3626s1c1
DNA Barcode:
CCCAACAAAGTCCTACATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsf (18195)
Gene Description:
N-ethylmaleimide sensitive fusion protein
Transcript:
RefSeq NM_008740.2 (NON-CURRENT)
Match location:
Position 3626 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACAAAGTCCTACATGAA
Hairpin Sequence:
5'-CCGG-CCCAACAAAGTCCTACATGAA-CTCGAG-TTCATGTAGGACTTTGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACAAAGTCCTACATGAACTCGAGTTCATGTAGGACTTTGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACAAAGTCCTACATGAACTCGAGTTCATGTAGGACTTTGTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000323687