Construct: shRNA TRCN0000101673

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177195.2-227s1c1
DNA Barcode:
CGGACAATTCCATCAAGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp8b5 (320571)
Gene Description:
ATPase, class I, type 8B, member 5
Transcript:
RefSeq NM_177195.2 (NON-CURRENT)
Match location:
Position 227 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101673)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACAATTCCATCAAGACAT
Hairpin Sequence:
5'-CCGG-CGGACAATTCCATCAAGACAT-CTCGAG-ATGTCTTGATGGAATTGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACAATTCCATCAAGACATCTCGAGATGTCTTGATGGAATTGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACAATTCCATCAAGACATCTCGAGATGTCTTGATGGAATTGTCCG-3'

Other clones with same target sequence:

(none)