Construct: shRNA TRCN0000101692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026993.1-585s1c1
DNA Barcode:
GTGCTGAAATCTTGGCTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddah1 (69219)
Gene Description:
dimethylarginine dimethylaminohydrolase 1
Transcript:
RefSeq NM_026993.1 (NON-CURRENT)
Match location:
Position 585 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTGAAATCTTGGCTGATA
Hairpin Sequence:
5'-CCGG-GTGCTGAAATCTTGGCTGATA-CTCGAG-TATCAGCCAAGATTTCAGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTGAAATCTTGGCTGATACTCGAGTATCAGCCAAGATTTCAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTGAAATCTTGGCTGATACTCGAGTATCAGCCAAGATTTCAGCAC-3'

Other clones with same target sequence:

  1. TRCN0000323689