Construct: shRNA TRCN0000101706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008102.2-414s1c1
DNA Barcode:
GCAGTACTTCACCAAGGGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gch1 (14528)
Gene Description:
GTP cyclohydrolase 1
Transcript:
RefSeq NM_008102.2 (NON-CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTACTTCACCAAGGGATA
Hairpin Sequence:
5'-CCGG-GCAGTACTTCACCAAGGGATA-CTCGAG-TATCCCTTGGTGAAGTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTACTTCACCAAGGGATACTCGAGTATCCCTTGGTGAAGTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTACTTCACCAAGGGATACTCGAGTATCCCTTGGTGAAGTACTGC-3'

Other clones with same target sequence:

  1. TRCN0000288139