Construct: shRNA TRCN0000101725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175650.2-4063s1c1
DNA Barcode:
CCGTATTCATACTGTGTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp13a5 (268878)
Gene Description:
ATPase type 13A5
Transcript:
RefSeq NM_175650.2 (NON-CURRENT)
Match location:
Position 4063 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTATTCATACTGTGTTGTT
Hairpin Sequence:
5'-CCGG-CCGTATTCATACTGTGTTGTT-CTCGAG-AACAACACAGTATGAATACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTATTCATACTGTGTTGTTCTCGAGAACAACACAGTATGAATACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTATTCATACTGTGTTGTTCTCGAGAACAACACAGTATGAATACGG-3'

Other clones with same target sequence:

(none)