Construct: shRNA TRCN0000101806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016722.2-453s1c1
DNA Barcode:
GCATGGATTTGACGAGTGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galns (50917)
Gene Description:
galactosamine (N-acetyl)-6-sulfate sulfatase
Transcript:
RefSeq NM_016722.2 (NON-CURRENT)
Match location:
Position 453 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGATTTGACGAGTGGTT
Hairpin Sequence:
5'-CCGG-GCATGGATTTGACGAGTGGTT-CTCGAG-AACCACTCGTCAAATCCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGATTTGACGAGTGGTTCTCGAGAACCACTCGTCAAATCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGATTTGACGAGTGGTTCTCGAGAACCACTCGTCAAATCCATGC-3'

Other clones with same target sequence:

  1. TRCN0000318119