Construct: shRNA TRCN0000101811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009726.2-2634s1c1
DNA Barcode:
GCCGTGTTATTGAAGGACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp7a (11977)
Gene Description:
ATPase, Cu++ transporting, alpha polypeptide
Transcript:
RefSeq NM_009726.2 (NON-CURRENT)
Match location:
Position 2634 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGTTATTGAAGGACATT
Hairpin Sequence:
5'-CCGG-GCCGTGTTATTGAAGGACATT-CTCGAG-AATGTCCTTCAATAACACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGTTATTGAAGGACATTCTCGAGAATGTCCTTCAATAACACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGTTATTGAAGGACATTCTCGAGAATGTCCTTCAATAACACGGC-3'

Other clones with same target sequence:

  1. TRCN0000316440