Construct: shRNA TRCN0000101812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009726.2-152s1c1
DNA Barcode:
CGGACCATTGAACAGCAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp7a (11977)
Gene Description:
ATPase, Cu++ transporting, alpha polypeptide
Transcript:
RefSeq NM_009726.2 (NON-CURRENT)
Match location:
Position 152 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACCATTGAACAGCAGATT
Hairpin Sequence:
5'-CCGG-CGGACCATTGAACAGCAGATT-CTCGAG-AATCTGCTGTTCAATGGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACCATTGAACAGCAGATTCTCGAGAATCTGCTGTTCAATGGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACCATTGAACAGCAGATTCTCGAGAATCTGCTGTTCAATGGTCCG-3'

Other clones with same target sequence:

  1. TRCN0000316361