Construct: shRNA TRCN0000101826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011596.1-1292s1c1
DNA Barcode:
GCCCTCTTACTGGTGTTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v0a2 (21871)
Gene Description:
ATPase, H+ transporting, lysosomal V0 subunit A2
Transcript:
RefSeq NM_011596.1 (NON-CURRENT)
Match location:
Position 1292 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCTTACTGGTGTTAAAT
Hairpin Sequence:
5'-CCGG-GCCCTCTTACTGGTGTTAAAT-CTCGAG-ATTTAACACCAGTAAGAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCTTACTGGTGTTAAATCTCGAGATTTAACACCAGTAAGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCTTACTGGTGTTAAATCTCGAGATTTAACACCAGTAAGAGGGC-3'

Other clones with same target sequence:

(none)