Construct: shRNA TRCN0000101874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009723.1-1720s1c1
DNA Barcode:
ACTGGCCTATTCTGTGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp2b2 (11941)
Gene Description:
ATPase, Ca++ transporting, plasma membrane 2
Transcript:
RefSeq NM_009723.1 (NON-CURRENT)
Match location:
Position 1720 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGCCTATTCTGTGAAGAA
Hairpin Sequence:
5'-CCGG-ACTGGCCTATTCTGTGAAGAA-CTCGAG-TTCTTCACAGAATAGGCCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGCCTATTCTGTGAAGAACTCGAGTTCTTCACAGAATAGGCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGCCTATTCTGTGAAGAACTCGAGTTCTTCACAGAATAGGCCAGT-3'

Other clones with same target sequence:

(none)