Construct: shRNA TRCN0000101891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013477.2-377s1c1
DNA Barcode:
GCCACATGAGAAACCATGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v0d1 (11972)
Gene Description:
ATPase, H+ transporting, lysosomal V0 subunit D1
Transcript:
RefSeq NM_013477.2 (NON-CURRENT)
Match location:
Position 377 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATGAGAAACCATGCTT
Hairpin Sequence:
5'-CCGG-GCCACATGAGAAACCATGCTT-CTCGAG-AAGCATGGTTTCTCATGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATGAGAAACCATGCTTCTCGAGAAGCATGGTTTCTCATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATGAGAAACCATGCTTCTCGAGAAGCATGGTTTCTCATGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000332629