Construct: shRNA TRCN0000101989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172499.1-163s1c1
DNA Barcode:
GTAGGCTTCTTGGACCTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mfsd9 (211798)
Gene Description:
major facilitator superfamily domain containing 9
Transcript:
RefSeq NM_172499.1 (NON-CURRENT)
Match location:
Position 163 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGGCTTCTTGGACCTGTTT
Hairpin Sequence:
5'-CCGG-GTAGGCTTCTTGGACCTGTTT-CTCGAG-AAACAGGTCCAAGAAGCCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGGCTTCTTGGACCTGTTTCTCGAGAAACAGGTCCAAGAAGCCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGGCTTCTTGGACCTGTTTCTCGAGAAACAGGTCCAAGAAGCCTAC-3'

Other clones with same target sequence:

(none)