Construct: shRNA TRCN0000101992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029937.1-663s1c1
DNA Barcode:
GACCCTGAAATTCATGCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec14l3 (380683)
Gene Description:
SEC14-like lipid binding 3
Transcript:
RefSeq NM_001029937.1 (NON-CURRENT)
Match location:
Position 663 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000101992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCTGAAATTCATGCTCAT
Hairpin Sequence:
5'-CCGG-GACCCTGAAATTCATGCTCAT-CTCGAG-ATGAGCATGAATTTCAGGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCTGAAATTCATGCTCATCTCGAGATGAGCATGAATTTCAGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCTGAAATTCATGCTCATCTCGAGATGAGCATGAATTTCAGGGTC-3'

Other clones with same target sequence:

(none)