Construct: shRNA TRCN0000102033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021376.1-812s1c1
DNA Barcode:
CTTTGGAATAATGGGACTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pmp47 (58177)
Gene Description:
peroxisomal integral membrane protein 47
Transcript:
RefSeq NM_021376.1 (NON-CURRENT)
Match location:
Position 812 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGAATAATGGGACTCTA
Hairpin Sequence:
5'-CCGG-CTTTGGAATAATGGGACTCTA-CTCGAG-TAGAGTCCCATTATTCCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGAATAATGGGACTCTACTCGAGTAGAGTCCCATTATTCCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGAATAATGGGACTCTACTCGAGTAGAGTCCCATTATTCCAAAG-3'

Other clones with same target sequence:

(none)