Construct: shRNA TRCN0000102040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177787.2-2086s1c1
DNA Barcode:
GCCCTCAAACTTAACTATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc15a5 (277898)
Gene Description:
solute carrier family 15, member 5
Transcript:
RefSeq NM_177787.2 (NON-CURRENT)
Match location:
Position 2086 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCAAACTTAACTATGTA
Hairpin Sequence:
5'-CCGG-GCCCTCAAACTTAACTATGTA-CTCGAG-TACATAGTTAAGTTTGAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCAAACTTAACTATGTACTCGAGTACATAGTTAAGTTTGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCAAACTTAACTATGTACTCGAGTACATAGTTAAGTTTGAGGGC-3'

Other clones with same target sequence:

(none)