Construct: shRNA TRCN0000102049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016714.2-344s1c1
DNA Barcode:
CAGAGCCGTAAAGAAGGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nup50 (18141)
Gene Description:
nucleoporin 50
Transcript:
RefSeq NM_016714.2 (CURRENT)
Match location:
Position 344 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGCCGTAAAGAAGGCAAA
Hairpin Sequence:
5'-CCGG-CAGAGCCGTAAAGAAGGCAAA-CTCGAG-TTTGCCTTCTTTACGGCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGCCGTAAAGAAGGCAAACTCGAGTTTGCCTTCTTTACGGCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGCCGTAAAGAAGGCAAACTCGAGTTTGCCTTCTTTACGGCTCTG-3'

Other clones with same target sequence:

  1. TRCN0000340811