Construct: shRNA TRCN0000102078

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172378.1-1651s1c1
DNA Barcode:
CCGTCGTCCTTTAATTGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc22a22 (210463)
Gene Description:
solute carrier family 22 (organic cation transporter), member 22
Transcript:
RefSeq NM_172378.1 (NON-CURRENT)
Match location:
Position 1651 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102078)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCGTCCTTTAATTGCTTT
Hairpin Sequence:
5'-CCGG-CCGTCGTCCTTTAATTGCTTT-CTCGAG-AAAGCAATTAAAGGACGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCGTCCTTTAATTGCTTTCTCGAGAAAGCAATTAAAGGACGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCGTCCTTTAATTGCTTTCTCGAGAAAGCAATTAAAGGACGACGG-3'

Other clones with same target sequence:

(none)