Construct: shRNA TRCN0000102103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176807.2-586s1c1
DNA Barcode:
GCCACTCATCTTTGACTTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Izumo1r (64931)
Gene Description:
IZUMO1 receptor, JUNO
Transcript:
RefSeq NM_176807.2 (NON-CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTCATCTTTGACTTGTA
Hairpin Sequence:
5'-CCGG-GCCACTCATCTTTGACTTGTA-CTCGAG-TACAAGTCAAAGATGAGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTCATCTTTGACTTGTACTCGAGTACAAGTCAAAGATGAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTCATCTTTGACTTGTACTCGAGTACAAGTCAAAGATGAGTGGC-3'

Other clones with same target sequence:

(none)