Construct: shRNA TRCN0000102143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010686.2-438s1c1
DNA Barcode:
GCTAGACTTCTGTTTGAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Laptm5 (16792)
Gene Description:
lysosomal-associated protein transmembrane 5
Transcript:
RefSeq NM_010686.2 (NON-CURRENT)
Match location:
Position 438 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGACTTCTGTTTGAGTAT
Hairpin Sequence:
5'-CCGG-GCTAGACTTCTGTTTGAGTAT-CTCGAG-ATACTCAAACAGAAGTCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGACTTCTGTTTGAGTATCTCGAGATACTCAAACAGAAGTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGACTTCTGTTTGAGTATCTCGAGATACTCAAACAGAAGTCTAGC-3'

Other clones with same target sequence:

(none)