Construct: shRNA TRCN0000102219

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199032.1-412s1c1
DNA Barcode:
CCAGTATGTTCACAAGGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep135 (381644)
Gene Description:
centrosomal protein 135
Transcript:
RefSeq NM_199032.1 (NON-CURRENT)
Match location:
Position 412 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102219)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTATGTTCACAAGGTCAA
Hairpin Sequence:
5'-CCGG-CCAGTATGTTCACAAGGTCAA-CTCGAG-TTGACCTTGTGAACATACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTATGTTCACAAGGTCAACTCGAGTTGACCTTGTGAACATACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTATGTTCACAAGGTCAACTCGAGTTGACCTTGTGAACATACTGG-3'

Other clones with same target sequence:

(none)