Construct: shRNA TRCN0000102244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023603.2-1837s1c1
DNA Barcode:
AGAAGAAAGTTACAGCAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sfpq (71514)
Gene Description:
splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
Transcript:
RefSeq NM_023603.2 (NON-CURRENT)
Match location:
Position 1837 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGAAAGTTACAGCAGGAT
Hairpin Sequence:
5'-CCGG-AGAAGAAAGTTACAGCAGGAT-CTCGAG-ATCCTGCTGTAACTTTCTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGAAAGTTACAGCAGGATCTCGAGATCCTGCTGTAACTTTCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGAAAGTTACAGCAGGATCTCGAGATCCTGCTGTAACTTTCTTCT-3'

Other clones with same target sequence:

(none)