Construct: shRNA TRCN0000102255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178610.2-2341s1c1
DNA Barcode:
CGTGACCTTGATTTGGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krr1 (52705)
Gene Description:
KRR1, small subunit (SSU) processome component, homolog (yeast)
Transcript:
RefSeq NM_178610.2 (NON-CURRENT)
Match location:
Position 2341 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGACCTTGATTTGGCTTTA
Hairpin Sequence:
5'-CCGG-CGTGACCTTGATTTGGCTTTA-CTCGAG-TAAAGCCAAATCAAGGTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGACCTTGATTTGGCTTTACTCGAGTAAAGCCAAATCAAGGTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGACCTTGATTTGGCTTTACTCGAGTAAAGCCAAATCAAGGTCACG-3'

Other clones with same target sequence:

(none)