Construct: shRNA TRCN0000102260

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030723.1-3395s1c1
DNA Barcode:
GCTGCTTTAACCATGTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pum2 (80913)
Gene Description:
pumilio RNA-binding family member 2
Transcript:
RefSeq NM_030723.1 (NON-CURRENT)
Match location:
Position 3395 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102260)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTTTAACCATGTTCAAA
Hairpin Sequence:
5'-CCGG-GCTGCTTTAACCATGTTCAAA-CTCGAG-TTTGAACATGGTTAAAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTTTAACCATGTTCAAACTCGAGTTTGAACATGGTTAAAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTTTAACCATGTTCAAACTCGAGTTTGAACATGGTTAAAGCAGC-3'

Other clones with same target sequence:

(none)