Construct: shRNA TRCN0000102271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026650.1-968s1c1
DNA Barcode:
CGGGACCTTAAAGGAGTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm28 (68272)
Gene Description:
RNA binding motif protein 28
Transcript:
RefSeq NM_026650.1 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGACCTTAAAGGAGTTCAT
Hairpin Sequence:
5'-CCGG-CGGGACCTTAAAGGAGTTCAT-CTCGAG-ATGAACTCCTTTAAGGTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGACCTTAAAGGAGTTCATCTCGAGATGAACTCCTTTAAGGTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGACCTTAAAGGAGTTCATCTCGAGATGAACTCCTTTAAGGTCCCG-3'

Other clones with same target sequence:

(none)