Construct: shRNA TRCN0000102334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133235.2-960s1c1
DNA Barcode:
AGACCTATGAGGCTTATGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Khdrbs2 (170771)
Gene Description:
KH domain containing, RNA binding, signal transduction associated 2
Transcript:
RefSeq NM_133235.2 (NON-CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCTATGAGGCTTATGATA
Hairpin Sequence:
5'-CCGG-AGACCTATGAGGCTTATGATA-CTCGAG-TATCATAAGCCTCATAGGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCTATGAGGCTTATGATACTCGAGTATCATAAGCCTCATAGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCTATGAGGCTTATGATACTCGAGTATCATAAGCCTCATAGGTCT-3'

Other clones with same target sequence:

(none)