Construct: shRNA TRCN0000102378
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_130881.2-1339s1c1
- DNA Barcode:
- GCCAGTTTGGTAAGACCCTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pabpc4 (230721)
- Gene Description:
- poly(A) binding protein, cytoplasmic 4
- Transcript:
- RefSeq NM_130881.2 (CURRENT)
- Match location:
- Position 1339 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000102378)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCAGTTTGGTAAGACCCTAA
- Hairpin Sequence:
-
5'-CCGG-GCCAGTTTGGTAAGACCCTAA-CTCGAG-TTAGGGTCTTACCAAACTGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAGTTTGGTAAGACCCTAACTCGAGTTAGGGTCTTACCAAACTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAGTTTGGTAAGACCCTAACTCGAGTTAGGGTCTTACCAAACTGGC-3'
Other clones with same target sequence:
(none)
