Construct: shRNA TRCN0000102390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198653.1-3241s1c1
DNA Barcode:
CCAAGGCTTACACAGAAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Iars2 (381314)
Gene Description:
isoleucine-tRNA synthetase 2, mitochondrial
Transcript:
RefSeq NM_198653.1 (NON-CURRENT)
Match location:
Position 3241 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGCTTACACAGAAAGTA
Hairpin Sequence:
5'-CCGG-CCAAGGCTTACACAGAAAGTA-CTCGAG-TACTTTCTGTGTAAGCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGCTTACACAGAAAGTACTCGAGTACTTTCTGTGTAAGCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGCTTACACAGAAAGTACTCGAGTACTTTCTGTGTAAGCCTTGG-3'

Other clones with same target sequence:

(none)