Construct: shRNA TRCN0000102422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009273.2-280s1c1
DNA Barcode:
AGAAGTGAACAAGTTTCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srp14 (20813)
Gene Description:
signal recognition particle 14
Transcript:
RefSeq NM_009273.2 (NON-CURRENT)
Match location:
Position 280 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGTGAACAAGTTTCAGAT
Hairpin Sequence:
5'-CCGG-AGAAGTGAACAAGTTTCAGAT-CTCGAG-ATCTGAAACTTGTTCACTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGTGAACAAGTTTCAGATCTCGAGATCTGAAACTTGTTCACTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGTGAACAAGTTTCAGATCTCGAGATCTGAAACTTGTTCACTTCT-3'

Other clones with same target sequence:

  1. TRCN0000327542