Construct: shRNA TRCN0000102459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207515.1-1547s1c1
DNA Barcode:
CAAAGAGACAAGCACTTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mbnl2 (105559)
Gene Description:
muscleblind like splicing factor 2
Transcript:
RefSeq NM_207515.1 (NON-CURRENT)
Match location:
Position 1547 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGAGACAAGCACTTGAAA
Hairpin Sequence:
5'-CCGG-CAAAGAGACAAGCACTTGAAA-CTCGAG-TTTCAAGTGCTTGTCTCTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGAGACAAGCACTTGAAACTCGAGTTTCAAGTGCTTGTCTCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGAGACAAGCACTTGAAACTCGAGTTTCAAGTGCTTGTCTCTTTG-3'

Other clones with same target sequence:

(none)