Construct: shRNA TRCN0000102480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027427.1-1783s1c1
DNA Barcode:
CTCCTTTGTCTCTGACATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taf15 (70439)
Gene Description:
TATA-box binding protein associated factor 15
Transcript:
RefSeq NM_027427.1 (NON-CURRENT)
Match location:
Position 1783 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTTTGTCTCTGACATGAT
Hairpin Sequence:
5'-CCGG-CTCCTTTGTCTCTGACATGAT-CTCGAG-ATCATGTCAGAGACAAAGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTTTGTCTCTGACATGATCTCGAGATCATGTCAGAGACAAAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTTTGTCTCTGACATGATCTCGAGATCATGTCAGAGACAAAGGAG-3'

Other clones with same target sequence:

(none)