Construct: shRNA TRCN0000102536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019402.1-692s1c1
DNA Barcode:
CCCTGTTCAGAGGAAGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pabpn1 (54196)
Gene Description:
poly(A) binding protein, nuclear 1
Transcript:
RefSeq NM_019402.1 (NON-CURRENT)
Match location:
Position 692 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTTCAGAGGAAGACAAA
Hairpin Sequence:
5'-CCGG-CCCTGTTCAGAGGAAGACAAA-CTCGAG-TTTGTCTTCCTCTGAACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTTCAGAGGAAGACAAACTCGAGTTTGTCTTCCTCTGAACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTTCAGAGGAAGACAAACTCGAGTTTGTCTTCCTCTGAACAGGG-3'

Other clones with same target sequence:

(none)