Construct: shRNA TRCN0000102582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153591.2-768s1c1
DNA Barcode:
GCTGCTATTCATTCCTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nars2 (244141)
Gene Description:
asparaginyl-tRNA synthetase 2 (mitochondrial)(putative)
Transcript:
RefSeq NM_153591.2 (NON-CURRENT)
Match location:
Position 768 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTATTCATTCCTACTTT
Hairpin Sequence:
5'-CCGG-GCTGCTATTCATTCCTACTTT-CTCGAG-AAAGTAGGAATGAATAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTATTCATTCCTACTTTCTCGAGAAAGTAGGAATGAATAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTATTCATTCCTACTTTCTCGAGAAAGTAGGAATGAATAGCAGC-3'

Other clones with same target sequence:

(none)