Construct: shRNA TRCN0000102704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_359315.1-891s1c1
DNA Barcode:
CAACCATAACTCGTTCCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rhoh (74734)
Gene Description:
ras homolog family member H
Transcript:
RefSeq XM_359315.1 (NON-CURRENT)
Match location:
Position 891 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCATAACTCGTTCCTGAA
Hairpin Sequence:
5'-CCGG-CAACCATAACTCGTTCCTGAA-CTCGAG-TTCAGGAACGAGTTATGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCATAACTCGTTCCTGAACTCGAGTTCAGGAACGAGTTATGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCATAACTCGTTCCTGAACTCGAGTTCAGGAACGAGTTATGGTTG-3'

Other clones with same target sequence:

(none)