Construct: shRNA TRCN0000102716

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172711.1-1811s1c1
DNA Barcode:
CCCAGACAACTGTATGAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Guf1 (231279)
Gene Description:
GUF1 homolog, GTPase
Transcript:
RefSeq NM_172711.1 (NON-CURRENT)
Match location:
Position 1811 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102716)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGACAACTGTATGAGATA
Hairpin Sequence:
5'-CCGG-CCCAGACAACTGTATGAGATA-CTCGAG-TATCTCATACAGTTGTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGACAACTGTATGAGATACTCGAGTATCTCATACAGTTGTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGACAACTGTATGAGATACTCGAGTATCTCATACAGTTGTCTGGG-3'

Other clones with same target sequence:

(none)