Construct: shRNA TRCN0000102812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133717.1-271s1c1
DNA Barcode:
GATCGAGGATGTGAGGAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab43 (69834)
Gene Description:
RAB43, member RAS oncogene family
Transcript:
RefSeq NM_133717.1 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCGAGGATGTGAGGAAGTA
Hairpin Sequence:
5'-CCGG-GATCGAGGATGTGAGGAAGTA-CTCGAG-TACTTCCTCACATCCTCGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCGAGGATGTGAGGAAGTACTCGAGTACTTCCTCACATCCTCGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCGAGGATGTGAGGAAGTACTCGAGTACTTCCTCACATCCTCGATC-3'

Other clones with same target sequence:

  1. TRCN0000318322