Construct: shRNA TRCN0000102822

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153082.2-277s1c1
DNA Barcode:
GCGCTACTGTGAGAAGAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajc27 (217378)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C27
Transcript:
RefSeq NM_153082.2 (NON-CURRENT)
Match location:
Position 277 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102822)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTACTGTGAGAAGAGATT
Hairpin Sequence:
5'-CCGG-GCGCTACTGTGAGAAGAGATT-CTCGAG-AATCTCTTCTCACAGTAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTACTGTGAGAAGAGATTCTCGAGAATCTCTTCTCACAGTAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTACTGTGAGAAGAGATTCTCGAGAATCTCTTCTCACAGTAGCGC-3'

Other clones with same target sequence:

(none)