Construct: shRNA TRCN0000102844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023868.1-6300s1c1
DNA Barcode:
GCTGCCAAAGACCTACACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ryr2 (20191)
Gene Description:
ryanodine receptor 2, cardiac
Transcript:
RefSeq NM_023868.1 (NON-CURRENT)
Match location:
Position 6300 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCCAAAGACCTACACTAT
Hairpin Sequence:
5'-CCGG-GCTGCCAAAGACCTACACTAT-CTCGAG-ATAGTGTAGGTCTTTGGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCCAAAGACCTACACTATCTCGAGATAGTGTAGGTCTTTGGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCCAAAGACCTACACTATCTCGAGATAGTGTAGGTCTTTGGCAGC-3'

Other clones with same target sequence:

(none)