Construct: shRNA TRCN0000102876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008069.3-227s1c1
DNA Barcode:
CGGAAGTGAATATGGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabrb1 (14400)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit beta 1
Transcript:
RefSeq NM_008069.3 (NON-CURRENT)
Match location:
Position 227 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGTGAATATGGATTATA
Hairpin Sequence:
5'-CCGG-CGGAAGTGAATATGGATTATA-CTCGAG-TATAATCCATATTCACTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGTGAATATGGATTATACTCGAGTATAATCCATATTCACTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGTGAATATGGATTATACTCGAGTATAATCCATATTCACTTCCG-3'

Other clones with same target sequence:

(none)