Construct: shRNA TRCN0000102880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_130497.2-5104s1c1
DNA Barcode:
CCGACATGGTTCAGAGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ryr3 (20192)
Gene Description:
ryanodine receptor 3
Transcript:
RefSeq XM_130497.2 (NON-CURRENT)
Match location:
Position 5104 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACATGGTTCAGAGAGAAA
Hairpin Sequence:
5'-CCGG-CCGACATGGTTCAGAGAGAAA-CTCGAG-TTTCTCTCTGAACCATGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACATGGTTCAGAGAGAAACTCGAGTTTCTCTCTGAACCATGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACATGGTTCAGAGAGAAACTCGAGTTTCTCTCTGAACCATGTCGG-3'

Other clones with same target sequence:

(none)