Construct: shRNA TRCN0000102882

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_130497.2-3676s1c1
DNA Barcode:
GCGATGGTGTTCTTTGTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ryr3 (20192)
Gene Description:
ryanodine receptor 3
Transcript:
RefSeq XM_130497.2 (NON-CURRENT)
Match location:
Position 3676 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102882)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATGGTGTTCTTTGTTCTT
Hairpin Sequence:
5'-CCGG-GCGATGGTGTTCTTTGTTCTT-CTCGAG-AAGAACAAAGAACACCATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATGGTGTTCTTTGTTCTTCTCGAGAAGAACAAAGAACACCATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATGGTGTTCTTTGTTCTTCTCGAGAAGAACAAAGAACACCATCGC-3'

Other clones with same target sequence:

(none)