Construct: shRNA TRCN0000102950
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010251.1-2321s1c1
- DNA Barcode:
- GCTAGAAAGTGAACCATCTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gabra4 (14397)
- Gene Description:
- gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4
- Transcript:
- RefSeq NM_010251.1 (NON-CURRENT)
- Match location:
- Position 2321 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000102950)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTAGAAAGTGAACCATCTTT
- Hairpin Sequence:
-
5'-CCGG-GCTAGAAAGTGAACCATCTTT-CTCGAG-AAAGATGGTTCACTTTCTAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTAGAAAGTGAACCATCTTTCTCGAGAAAGATGGTTCACTTTCTAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTAGAAAGTGAACCATCTTTCTCGAGAAAGATGGTTCACTTTCTAGC-3'
Other clones with same target sequence:
(none)
