Construct: shRNA TRCN0000102953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010251.1-606s1c1
DNA Barcode:
CAGAACTCAAAGGACGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabra4 (14397)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4
Transcript:
RefSeq NM_010251.1 (NON-CURRENT)
Match location:
Position 606 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAACTCAAAGGACGAGAAA
Hairpin Sequence:
5'-CCGG-CAGAACTCAAAGGACGAGAAA-CTCGAG-TTTCTCGTCCTTTGAGTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAACTCAAAGGACGAGAAACTCGAGTTTCTCGTCCTTTGAGTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAACTCAAAGGACGAGAAACTCGAGTTTCTCGTCCTTTGAGTTCTG-3'

Other clones with same target sequence:

(none)