Construct: shRNA TRCN0000102975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008076.2-1695s1c1
DNA Barcode:
TGGTGGATACATGGACCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabrr2 (14409)
Gene Description:
gamma-aminobutyric acid (GABA) C receptor, subunit rho 2
Transcript:
RefSeq NM_008076.2 (NON-CURRENT)
Match location:
Position 1695 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGGATACATGGACCTAAT
Hairpin Sequence:
5'-CCGG-TGGTGGATACATGGACCTAAT-CTCGAG-ATTAGGTCCATGTATCCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGGATACATGGACCTAATCTCGAGATTAGGTCCATGTATCCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGGATACATGGACCTAATCTCGAGATTAGGTCCATGTATCCACCA-3'

Other clones with same target sequence:

(none)