Construct: shRNA TRCN0000102982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008070.2-1181s1c1
DNA Barcode:
GCCATCAATTCTGATTACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gabrb2 (14401)
Gene Description:
gamma-aminobutyric acid (GABA) A receptor, subunit beta 2
Transcript:
RefSeq NM_008070.2 (NON-CURRENT)
Match location:
Position 1181 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCAATTCTGATTACCAT
Hairpin Sequence:
5'-CCGG-GCCATCAATTCTGATTACCAT-CTCGAG-ATGGTAATCAGAATTGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCAATTCTGATTACCATCTCGAGATGGTAATCAGAATTGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCAATTCTGATTACCATCTCGAGATGGTAATCAGAATTGATGGC-3'

Other clones with same target sequence:

(none)