Construct: shRNA TRCN0000102998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011510.2-2468s1c1
DNA Barcode:
CCTTCGAGAGTCCCTTCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcc8 (20927)
Gene Description:
ATP-binding cassette, sub-family C (CFTR/MRP), member 8
Transcript:
RefSeq NM_011510.2 (NON-CURRENT)
Match location:
Position 2468 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000102998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGAGAGTCCCTTCAATA
Hairpin Sequence:
5'-CCGG-CCTTCGAGAGTCCCTTCAATA-CTCGAG-TATTGAAGGGACTCTCGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGAGAGTCCCTTCAATACTCGAGTATTGAAGGGACTCTCGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGAGAGTCCCTTCAATACTCGAGTATTGAAGGGACTCTCGAAGG-3'

Other clones with same target sequence:

(none)