Construct: shRNA TRCN0000103124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175140.3-1134s1c1
DNA Barcode:
CCGCCTCAGTACCTACACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chst8 (68947)
Gene Description:
carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8
Transcript:
RefSeq NM_175140.3 (NON-CURRENT)
Match location:
Position 1134 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCCTCAGTACCTACACCAA
Hairpin Sequence:
5'-CCGG-CCGCCTCAGTACCTACACCAA-CTCGAG-TTGGTGTAGGTACTGAGGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCCTCAGTACCTACACCAACTCGAGTTGGTGTAGGTACTGAGGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCCTCAGTACCTACACCAACTCGAGTTGGTGTAGGTACTGAGGCGG-3'

Other clones with same target sequence:

(none)